human lung asc cell line nci h596 Search Results


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ATCC lung carcinoma nci h596
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ATCC h596 atcc htb 178 human
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ATCC human lung cancer cell line h596
Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, <t>H596,</t> OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.
Human Lung Cancer Cell Line H596, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC human nsclc lines
Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, <t>H596,</t> OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.
Human Nsclc Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC human lung cancer cell lines
Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, <t>H596,</t> OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.
Human Lung Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC humannsclccells
Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, <t>H596,</t> OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.
Humannsclccells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC 2 h358 crl 5807 nsclc bronchioloalveolar 3 h596 htb
Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, <t>H596,</t> OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.
2 H358 Crl 5807 Nsclc Bronchioloalveolar 3 H596 Htb, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, H596, OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.

Journal: Gene therapy

Article Title: Potential for Enhanced Therapeutic Activity of Biological Cancer Therapies with Doxycycline Combination

doi: 10.1038/gt.2012.96

Figure Lengend Snippet: Doxycycline enhances viral replication and gene expression after treatment of multiple tumor cell lines with oncolytic vaccinia strain vvDD. (A) Different tumor cell lines were infected (Multiplicity of Infection of 1.0 PFU/cell) with oncolytic vaccinia strain vvDD expressing luciferase, with and without the presence of 1μg/ml doxycycline. Viral gene expression was determined at indicated times by bioluminescence imaging. Cell lines HeLa, HT29, OVCAR3, H596, OVCAR8, MDA-MB-231, DLD1 and HCT116 all displayed significantly increased gene expression at 48h after infection, p<0.05) (B) Viral plaque assays confirmed the increased gene expression correlated with viral replication for selected cell lines at 48h. (C) The same gene expression assay was performed on the non-tumor (immortalized, non-transformed) cell line MRC-5. (C) Viral replication was also determined by plaque assay r in human CIK cells.

Article Snippet: Human ovarian cancer cell lines Skov3, cervical cancer cell line Hela, human colon cancer cell lines DLD1, HCT116 and HT29, human lung cancer cell line H596 and human normal lung fibroblast cell line MRC5 were obtained from American Type Culture Collection (ATCC).

Techniques: Gene Expression, Infection, Expressing, Luciferase, Imaging, Transformation Assay, Plaque Assay